Determination of Gene Frequency of 10 New STR Loci in Turkey: Experimental Research

Objective: Short tandem repeats (STRs) have been widely used in human identification forensics. Evaluation and design of new markers are useful tools to obtain additional information complete conventional analysis. Also, it will be an alternative way confirm the results problematic cases (complex kinship, degraded samples, etc.). Increasing number loci result reducing risk adventitious matches countries that a national DNA database. In this study, we investigated allele frequencies 10 non-Combined Index System STR (D7S1517, D3S1744, D12S391, D2S1360, D6S474, D4S2366, D8S1132, D5S2500, D21S2055, D10S2325) by using Investigator® HDplex kit. Material Methods: DNAs were extracted from 100 blood samples QIAmp Mini Kit (Qiagen). amplified according Polymerase chain reaction products separated with ABI 3130 Genetic Analyzer analyzed GeneMapper IDX software. Forensic population parameters estimated Promega PowerStats Excel sheet. Allele frequencies, p-values for Hardy-Weinberg equilibrium, differentiation based on (Fst) calculated Arlequin ver.3.5. Results: We observed all showed high power discrimination. For comparison, found statistically significant differences between Türkiye African, East Asian, American populations at couple loci. Besides, there was no variation Turkish European as expected. Conclusion: This study provides data forensic laboratories kit can support existing